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Cortisol, Free, 24 Hour Urine Test


* This is not a home test kit. You will need to visit a local lab for testing.


This urine test helps with the initial evaluation of patients with suspected Cushing syndrome (CS). Cushing’s syndrome (a.k.a hypercortisolism) is a condition that occurs from exposure to high cortisol levels for an extended period of time.

Evaluating a 24-hour urine specimen combines the production of cortisol for an entire day and is not affected by cortisol-binding globulin (CBG) levels even though serum cortisol levels fluctuate unpredictably and are strongly dependent on CBG levels. Urinary cortisol shows the portion of serum-free cortisol filtered by the kidney, and corresponds well with cortisol secretion rate.

Some symptoms of CS include high blood pressure, weight gain in the abdominial area, but with thin arms and legs, reddish stretch marks, a round red face, a fat lump between the shoulders, muscle weakness, bone weakness, acne and fragile skin. Women also may have more hair and irregular menstruation as a result of CS.

Recommended For:

Diagnosing Cushing syndrome (CS)

Special Notes:

Patients must urinate at 8 a.m. and discard the specimen, then collect all urine in a 24-hour period, ending with the final collection at 8 a.m. the next morning.

Tests Included:

Cortisol, Free, 24 Hour Urine (14534)

Estimated Turn Around For Results:

2-10 Business Days

For an exact turnaround time for results, please contact us at Personalabs and we will contact the lab on your behalf as turnaround times vary depending on testing location or lab testing provider.

  • Step 1 - Order Your Test

    Create an account, choose your tests, checkout securely, generate your lab order.

  • Step 2 - Generate & Print Out Lab Order

    Our physician network will approve your order within 2-4hrs. Some lab orders can take 24 hours for approval.

  • Step 3 - Visit the Lab

    The test price decides which lab you will visit: Quest or Labcorp. Find a location in your area.

  • Step 4 - Get Your Results

    Results can be provided in as little as 24-48 hrs. For more complex tests, please contact us for turn around times.

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