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Vitamin D 25-Hydroxy Blood Test

* This is not a home test kit. You will need to visit a local lab for testing.

Also Known as

25-hydroxyvitamin D, 25(OH)D, Calcidiol Test, Cholecalciferol Test, Ergocalciferol Test, Vitamin D2 test, Vitamin D3 test

The Purpose of a Vitamin D 25-Hydroxy Test.

If you’re lacking in vitamin D, you’re not alone—about 35% of American adults don’t get enough of this essential vitamin. That’s partly because vitamin D can only be produced by the body when it synthesizes the sun’s rays through the skin, or when you consume foods or supplements with vitamin D. So if you don’t get enough sun exposure or eat a diet lacking in the right foods, you may be missing out on vitamin D. And a deficiency can be serious because you need vitamin D for bone, nerve, and muscle health. That’s why you want to check your vitamin D 25-hydroxy levels.

Vitamin D (25-hydroxy-vitamin D) (17306)
Vitamin D (25-hydroxy-vitamin D) (081950)

What Does the 25-Hydroxy Vitamin D Test Measure?

The test measures your levels of vitamin D 25-hydroxy, which is the form of vitamin D after it’s been processed by your liver.

Who Should Take a 25-Hydroxy Vitamin D Blood Test?

Testing may be advised if you:

  • Have a medical condition such as celiac disease, Crohn’s disease, or obesity.
  • Avoid sun exposure or regularly wear sunscreen.
  • Are an older adult; advanced age makes it more difficult for your body to get vitamin D from sunshine.
  • Have a liver disorder that prevents you from converting vitamin D to the 25-hydroxy form.
  • Experience symptoms associated with vitamin D deficiency, including bone pain or weakness, weak muscles, or poor bone density.

Vitamin D 25-Hydroxy Test Preparation

You don’t have to take any special steps before a 25-hydroxy vitamin D blood test.

Vitamin D 25-Hydroxy Results

Vitamin D hydroxy-25 levels can range from 20 to 50 nanograms per milliliter (ng/mL), depending on the test, to be considered normal. If your levels are too low, you may need to consider adding supplements or vitamin D-rich foods such as eggs, fish, or fortified milk to your daily diet. If you need guidance or support, you can talk with one of our board-certified physicians during a telehealth appointment.

Where Can I Get a Vitamin D 25-Hydroxy Blood Test Near Me?

With more than 400 tests in our online store and more than 4,000 labs across the country to choose from, it couldn’t be easier to get a 25-hydroxy vitamin D test. Trust Personalabs to deliver the power of healthcare and put it in your hands.

Here’s how the process works:

Step 1: Choose your test and download the lab order after purchasing it. (You may not need a doctor’s approval to make the purchase, depending on the state where you live.)

Step 2: Use the lab locator tool to find a facility for your walk-in or appointment testing.

Step 3: Bring the test order with you when you get your blood drawn.

Step 4: Find your results report in your private Personalabs account within 2 to 10 business days. (If needed, contact us to request a specific turnaround time from the lab.)

Personalabs gives you the tools to take charge of your health. Browse our full range of tests today.

Additional information


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Results can be provided in as little as 24-48 hrs. For more complex tests, please contact us for turn around times.


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